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sgrnas targeting adam10  (Addgene inc)


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    Addgene inc sgrnas targeting adam10
    A , Schematic of the CRISPR-Cas9 screen. B , Volcano plot of genes from the screen, ranked by fold change and adjusted P value. C , Representative flow cytometry dot plots of GW01-induced syncytia between A549-spike and FCER1G -KO (FcRγ-KO) THP-1 clones versus non-targeting control (NT), and quantification of syncytia(right inset)(mean ± SEM, n=3, * P <0.05, **** P <0.0001). D , Representative plots A549-spike fusion with <t>ADAM10-KO</t> THP-1 clones versus control and quantification of syncytia. E , Rescue of FcRγ: representative plots for FcRγ-KO-LVX (empty lentiviral vector), FcRγ-KO-FcRγ (FcRγ cDNA), and NT-LVX; with quantification of syncythia. F , Rescue of ADAM10: representative plots for ADAM10-KO-LVX (empty vector), ADAM10-KO-ADAM10 (ADAM10 cDNA), and NT-LVX, along with quantification of syncytia. G , Effect of CD64 or CD32 blocking antibodies on GW01-dependent syncytium formation measured by NanoLuc assay. H , Effect of the ADAM10 inhibitor GI254023X on GW01-dependent syncytium formation. I , Representative fluorescence images of syncytia (yellow merge) between THP-1-mCherry (red) and A549-spike (green; CFSE) at 3 hour post co-culture, with or without GI254023X. Scale bar, 100 μm. Data are presented as mean ± SEM, n=3, * P <0.05, *** P <0.001, **** P <0.0001 by one-way ANOVA.
    Sgrnas Targeting Adam10, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sgrnas+targeting+adam10/bio_rxiv__64898__2026__02__11__705426-195-0-9?v=Addgene+inc
    Average 94 stars, based on 52 article reviews
    sgrnas targeting adam10 - by Bioz Stars, 2026-07
    94/100 stars

    Images

    1) Product Images from "Antibody-Dependent Heterotypic Syncytia Drive COVID-19 Inflammation and Disease Progression"

    Article Title: Antibody-Dependent Heterotypic Syncytia Drive COVID-19 Inflammation and Disease Progression

    Journal: bioRxiv

    doi: 10.64898/2026.02.11.705426

    A , Schematic of the CRISPR-Cas9 screen. B , Volcano plot of genes from the screen, ranked by fold change and adjusted P value. C , Representative flow cytometry dot plots of GW01-induced syncytia between A549-spike and FCER1G -KO (FcRγ-KO) THP-1 clones versus non-targeting control (NT), and quantification of syncytia(right inset)(mean ± SEM, n=3, * P <0.05, **** P <0.0001). D , Representative plots A549-spike fusion with ADAM10-KO THP-1 clones versus control and quantification of syncytia. E , Rescue of FcRγ: representative plots for FcRγ-KO-LVX (empty lentiviral vector), FcRγ-KO-FcRγ (FcRγ cDNA), and NT-LVX; with quantification of syncythia. F , Rescue of ADAM10: representative plots for ADAM10-KO-LVX (empty vector), ADAM10-KO-ADAM10 (ADAM10 cDNA), and NT-LVX, along with quantification of syncytia. G , Effect of CD64 or CD32 blocking antibodies on GW01-dependent syncytium formation measured by NanoLuc assay. H , Effect of the ADAM10 inhibitor GI254023X on GW01-dependent syncytium formation. I , Representative fluorescence images of syncytia (yellow merge) between THP-1-mCherry (red) and A549-spike (green; CFSE) at 3 hour post co-culture, with or without GI254023X. Scale bar, 100 μm. Data are presented as mean ± SEM, n=3, * P <0.05, *** P <0.001, **** P <0.0001 by one-way ANOVA.
    Figure Legend Snippet: A , Schematic of the CRISPR-Cas9 screen. B , Volcano plot of genes from the screen, ranked by fold change and adjusted P value. C , Representative flow cytometry dot plots of GW01-induced syncytia between A549-spike and FCER1G -KO (FcRγ-KO) THP-1 clones versus non-targeting control (NT), and quantification of syncytia(right inset)(mean ± SEM, n=3, * P <0.05, **** P <0.0001). D , Representative plots A549-spike fusion with ADAM10-KO THP-1 clones versus control and quantification of syncytia. E , Rescue of FcRγ: representative plots for FcRγ-KO-LVX (empty lentiviral vector), FcRγ-KO-FcRγ (FcRγ cDNA), and NT-LVX; with quantification of syncythia. F , Rescue of ADAM10: representative plots for ADAM10-KO-LVX (empty vector), ADAM10-KO-ADAM10 (ADAM10 cDNA), and NT-LVX, along with quantification of syncytia. G , Effect of CD64 or CD32 blocking antibodies on GW01-dependent syncytium formation measured by NanoLuc assay. H , Effect of the ADAM10 inhibitor GI254023X on GW01-dependent syncytium formation. I , Representative fluorescence images of syncytia (yellow merge) between THP-1-mCherry (red) and A549-spike (green; CFSE) at 3 hour post co-culture, with or without GI254023X. Scale bar, 100 μm. Data are presented as mean ± SEM, n=3, * P <0.05, *** P <0.001, **** P <0.0001 by one-way ANOVA.

    Techniques Used: CRISPR, Flow Cytometry, Clone Assay, Control, Plasmid Preparation, Blocking Assay, Fluorescence, Co-Culture Assay

    A , Representative flow cytometry plots showing effect of GI254023X (ADAM10 inhibitor) on GW01-dependent infection of THP-1. Histogram (right) shows effect of GI254023X concentration on SARS-CoV-2 infection of THP-1. B , Representative flow cytometry dot plots showing effect of knockout of ADAM10 or FcRγ of THP-1 on GW01-dependent direct infection of THP-1. C , Representative flow cytometry plots showing effect of indicated dose of camostat, hydroxychloroquine (HCQ), and E64d on GW01-dependent infection of THP-1. D , Dose-dependent effect of camostat, hydroxychloroquine (HCQ), and E64d on GW01-dependent SARS-CoV-2 infection of THP-1 (mean ± SEM, n=3, **** P <0.0001, compared with camostat group of same dose, by two-way ANOVA).
    Figure Legend Snippet: A , Representative flow cytometry plots showing effect of GI254023X (ADAM10 inhibitor) on GW01-dependent infection of THP-1. Histogram (right) shows effect of GI254023X concentration on SARS-CoV-2 infection of THP-1. B , Representative flow cytometry dot plots showing effect of knockout of ADAM10 or FcRγ of THP-1 on GW01-dependent direct infection of THP-1. C , Representative flow cytometry plots showing effect of indicated dose of camostat, hydroxychloroquine (HCQ), and E64d on GW01-dependent infection of THP-1. D , Dose-dependent effect of camostat, hydroxychloroquine (HCQ), and E64d on GW01-dependent SARS-CoV-2 infection of THP-1 (mean ± SEM, n=3, **** P <0.0001, compared with camostat group of same dose, by two-way ANOVA).

    Techniques Used: Flow Cytometry, Infection, Concentration Assay, Knock-Out

    A , scRNA-seq clustering of postmortem lung tissue from patients with COVID-19. B , SARS-CoV-2 RNA expression across clusters. C , Heatmap of SARS-CoV-2 gene expression and cluster marker genes for each population. D , Expression of CD163, FCER1G , and ADAM10 . E , Heatmap of pathway enrichment in each cell population. F , ADAM10 expression in indicated cell types across clinical severity groups. G , Correlation of ADAM10 with IL6, IL1B , and TNF expression in doublets from postmortem COVID-19 lung tissue.
    Figure Legend Snippet: A , scRNA-seq clustering of postmortem lung tissue from patients with COVID-19. B , SARS-CoV-2 RNA expression across clusters. C , Heatmap of SARS-CoV-2 gene expression and cluster marker genes for each population. D , Expression of CD163, FCER1G , and ADAM10 . E , Heatmap of pathway enrichment in each cell population. F , ADAM10 expression in indicated cell types across clinical severity groups. G , Correlation of ADAM10 with IL6, IL1B , and TNF expression in doublets from postmortem COVID-19 lung tissue.

    Techniques Used: RNA Expression, Gene Expression, Marker, Expressing



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    sgrnas targeting adam10  (Addgene inc)
    94
    Addgene inc sgrnas targeting adam10
    A , Schematic of the CRISPR-Cas9 screen. B , Volcano plot of genes from the screen, ranked by fold change and adjusted P value. C , Representative flow cytometry dot plots of GW01-induced syncytia between A549-spike and FCER1G -KO (FcRγ-KO) THP-1 clones versus non-targeting control (NT), and quantification of syncytia(right inset)(mean ± SEM, n=3, * P <0.05, **** P <0.0001). D , Representative plots A549-spike fusion with <t>ADAM10-KO</t> THP-1 clones versus control and quantification of syncytia. E , Rescue of FcRγ: representative plots for FcRγ-KO-LVX (empty lentiviral vector), FcRγ-KO-FcRγ (FcRγ cDNA), and NT-LVX; with quantification of syncythia. F , Rescue of ADAM10: representative plots for ADAM10-KO-LVX (empty vector), ADAM10-KO-ADAM10 (ADAM10 cDNA), and NT-LVX, along with quantification of syncytia. G , Effect of CD64 or CD32 blocking antibodies on GW01-dependent syncytium formation measured by NanoLuc assay. H , Effect of the ADAM10 inhibitor GI254023X on GW01-dependent syncytium formation. I , Representative fluorescence images of syncytia (yellow merge) between THP-1-mCherry (red) and A549-spike (green; CFSE) at 3 hour post co-culture, with or without GI254023X. Scale bar, 100 μm. Data are presented as mean ± SEM, n=3, * P <0.05, *** P <0.001, **** P <0.0001 by one-way ANOVA.
    Sgrnas Targeting Adam10, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sgrnas+targeting+adam10/bio_rxiv__64898__2026__02__11__705426-195-0-9?v=Addgene+inc
    Average 94 stars, based on 1 article reviews
    sgrnas targeting adam10 - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    A , Schematic of the CRISPR-Cas9 screen. B , Volcano plot of genes from the screen, ranked by fold change and adjusted P value. C , Representative flow cytometry dot plots of GW01-induced syncytia between A549-spike and FCER1G -KO (FcRγ-KO) THP-1 clones versus non-targeting control (NT), and quantification of syncytia(right inset)(mean ± SEM, n=3, * P <0.05, **** P <0.0001). D , Representative plots A549-spike fusion with ADAM10-KO THP-1 clones versus control and quantification of syncytia. E , Rescue of FcRγ: representative plots for FcRγ-KO-LVX (empty lentiviral vector), FcRγ-KO-FcRγ (FcRγ cDNA), and NT-LVX; with quantification of syncythia. F , Rescue of ADAM10: representative plots for ADAM10-KO-LVX (empty vector), ADAM10-KO-ADAM10 (ADAM10 cDNA), and NT-LVX, along with quantification of syncytia. G , Effect of CD64 or CD32 blocking antibodies on GW01-dependent syncytium formation measured by NanoLuc assay. H , Effect of the ADAM10 inhibitor GI254023X on GW01-dependent syncytium formation. I , Representative fluorescence images of syncytia (yellow merge) between THP-1-mCherry (red) and A549-spike (green; CFSE) at 3 hour post co-culture, with or without GI254023X. Scale bar, 100 μm. Data are presented as mean ± SEM, n=3, * P <0.05, *** P <0.001, **** P <0.0001 by one-way ANOVA.

    Journal: bioRxiv

    Article Title: Antibody-Dependent Heterotypic Syncytia Drive COVID-19 Inflammation and Disease Progression

    doi: 10.64898/2026.02.11.705426

    Figure Lengend Snippet: A , Schematic of the CRISPR-Cas9 screen. B , Volcano plot of genes from the screen, ranked by fold change and adjusted P value. C , Representative flow cytometry dot plots of GW01-induced syncytia between A549-spike and FCER1G -KO (FcRγ-KO) THP-1 clones versus non-targeting control (NT), and quantification of syncytia(right inset)(mean ± SEM, n=3, * P <0.05, **** P <0.0001). D , Representative plots A549-spike fusion with ADAM10-KO THP-1 clones versus control and quantification of syncytia. E , Rescue of FcRγ: representative plots for FcRγ-KO-LVX (empty lentiviral vector), FcRγ-KO-FcRγ (FcRγ cDNA), and NT-LVX; with quantification of syncythia. F , Rescue of ADAM10: representative plots for ADAM10-KO-LVX (empty vector), ADAM10-KO-ADAM10 (ADAM10 cDNA), and NT-LVX, along with quantification of syncytia. G , Effect of CD64 or CD32 blocking antibodies on GW01-dependent syncytium formation measured by NanoLuc assay. H , Effect of the ADAM10 inhibitor GI254023X on GW01-dependent syncytium formation. I , Representative fluorescence images of syncytia (yellow merge) between THP-1-mCherry (red) and A549-spike (green; CFSE) at 3 hour post co-culture, with or without GI254023X. Scale bar, 100 μm. Data are presented as mean ± SEM, n=3, * P <0.05, *** P <0.001, **** P <0.0001 by one-way ANOVA.

    Article Snippet: sgRNAs targeting ADAM10 and FCER1G were cloned into pMCB320 (Addgene, 89359) and used to transduce THP-1-Cas9 cells (third-generation packaging).

    Techniques: CRISPR, Flow Cytometry, Clone Assay, Control, Plasmid Preparation, Blocking Assay, Fluorescence, Co-Culture Assay

    A , Representative flow cytometry plots showing effect of GI254023X (ADAM10 inhibitor) on GW01-dependent infection of THP-1. Histogram (right) shows effect of GI254023X concentration on SARS-CoV-2 infection of THP-1. B , Representative flow cytometry dot plots showing effect of knockout of ADAM10 or FcRγ of THP-1 on GW01-dependent direct infection of THP-1. C , Representative flow cytometry plots showing effect of indicated dose of camostat, hydroxychloroquine (HCQ), and E64d on GW01-dependent infection of THP-1. D , Dose-dependent effect of camostat, hydroxychloroquine (HCQ), and E64d on GW01-dependent SARS-CoV-2 infection of THP-1 (mean ± SEM, n=3, **** P <0.0001, compared with camostat group of same dose, by two-way ANOVA).

    Journal: bioRxiv

    Article Title: Antibody-Dependent Heterotypic Syncytia Drive COVID-19 Inflammation and Disease Progression

    doi: 10.64898/2026.02.11.705426

    Figure Lengend Snippet: A , Representative flow cytometry plots showing effect of GI254023X (ADAM10 inhibitor) on GW01-dependent infection of THP-1. Histogram (right) shows effect of GI254023X concentration on SARS-CoV-2 infection of THP-1. B , Representative flow cytometry dot plots showing effect of knockout of ADAM10 or FcRγ of THP-1 on GW01-dependent direct infection of THP-1. C , Representative flow cytometry plots showing effect of indicated dose of camostat, hydroxychloroquine (HCQ), and E64d on GW01-dependent infection of THP-1. D , Dose-dependent effect of camostat, hydroxychloroquine (HCQ), and E64d on GW01-dependent SARS-CoV-2 infection of THP-1 (mean ± SEM, n=3, **** P <0.0001, compared with camostat group of same dose, by two-way ANOVA).

    Article Snippet: sgRNAs targeting ADAM10 and FCER1G were cloned into pMCB320 (Addgene, 89359) and used to transduce THP-1-Cas9 cells (third-generation packaging).

    Techniques: Flow Cytometry, Infection, Concentration Assay, Knock-Out

    A , scRNA-seq clustering of postmortem lung tissue from patients with COVID-19. B , SARS-CoV-2 RNA expression across clusters. C , Heatmap of SARS-CoV-2 gene expression and cluster marker genes for each population. D , Expression of CD163, FCER1G , and ADAM10 . E , Heatmap of pathway enrichment in each cell population. F , ADAM10 expression in indicated cell types across clinical severity groups. G , Correlation of ADAM10 with IL6, IL1B , and TNF expression in doublets from postmortem COVID-19 lung tissue.

    Journal: bioRxiv

    Article Title: Antibody-Dependent Heterotypic Syncytia Drive COVID-19 Inflammation and Disease Progression

    doi: 10.64898/2026.02.11.705426

    Figure Lengend Snippet: A , scRNA-seq clustering of postmortem lung tissue from patients with COVID-19. B , SARS-CoV-2 RNA expression across clusters. C , Heatmap of SARS-CoV-2 gene expression and cluster marker genes for each population. D , Expression of CD163, FCER1G , and ADAM10 . E , Heatmap of pathway enrichment in each cell population. F , ADAM10 expression in indicated cell types across clinical severity groups. G , Correlation of ADAM10 with IL6, IL1B , and TNF expression in doublets from postmortem COVID-19 lung tissue.

    Article Snippet: sgRNAs targeting ADAM10 and FCER1G were cloned into pMCB320 (Addgene, 89359) and used to transduce THP-1-Cas9 cells (third-generation packaging).

    Techniques: RNA Expression, Gene Expression, Marker, Expressing